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Since you can only use SMS if you already have an Authenticator App enabled, you should really think carefully about whether it’s worth adding a more easily stolen MFA after setting up a better option with your Authenticator App. Hackers have been able to intercept text messages or call up phone carriers to do a “SIM Swap” attack and take over phone numbers. If we can be transparent with you, SMS MFA is better than no MFA, but not by a lot. But these codes are still phishable, so if you lose your phone, this could lead to a predicament. With an Authenticator App, you’ll register a QR code or manually enter a key with an app on your phone or a password manager and then generate a new code for you to use every 30 seconds.Īs long as you have your phone or access to your password manager, you can make new codes and hackers will have a hard time getting access to your app. Plus the name makes us think we’re in a spy novel. The Authenticator App is an excellent choice that mixes flexibility with security. This method is the best way to protect your account because it is simple to use, on your own devices, backed up in the cloud, and, most importantly, phishing resistant. And to be clear, we’re never given any biometrics data from your device. Whenever you want to use the passkey, you authenticate using your biometric so that your device sends the passkey data to Discord. The way this works is when you register a passkey for Discord, you’ll protect it using your biometric (fingerprint or face) and the key itself lives on your device and in your provider’s cloud (e.g. FaceId, TouchId, Windows Hello) to gain access to your account. This is the latest in personal security, and also the most secure! With Security Keys, you’ll be able to use a passkey (ex. We’ll talk through each option and give you some info on the security of each one. You can choose any one of these options or any combo, but there are positives and negatives to each. There are three options to add MFA to your account: Security Keys, an Authenticator App, and SMS. Go through the list of accounts you've configured in the app, turning 2FA off and on for each one.Multi-Factor Authentication (MFA for short) is the best way to add an extra layer of security to your Discord account to make sure that only you have the ability to log in. To remove an account from Google Authenticator, tap and hold on it, then press the Trash Button (top right). It's always a good idea to check that the login you've swapped is working before moving on to the next one. In Authy, tap Add Account and then Scan QR Code. Click Set Up, and you'll eventually be shown a QR code, which you can scan using the Authy app. These days, Google prefers to use a prompt on your phone as the 2FA confirmation, but you'll find an authenticator app option further down the settings screen once 2FA is back in place. You'll be taken through the process of setting up 2FA on your account. With a Google account, for example, you need to open your account page on the web, select Security and 2-Step Verification, click Turn Off, confirm your choice, click 2-Step Verification again, and then click Get Started. Obviously, the exact process will depend on which accounts you use. Two-factor settings for a Google account. 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To get around this, you can use third-party file systems, for example, (free) or (14 days free) (Different devices may have different file explorers, and often, there is a problem when you can copy from the "Android/data" folder but can not paste files back into it. Go back to Files and copy your "saves" from a separate location back to "Android/data//files".Uninstall the game and install the new version run it once.Before uninstalling the game, go to the "Android/data//files" folder and copy the "saves" folder to a separate location on your phone.Mac ( Compressed ) - Mega, MixDrop, PixelDrainĪndroid v0.3.2 (Third Act) - Mega, MixDrop, PixelDrainĪndroid ( First and Second Acts ) - Mega, MixDrop, PixelDrain Windows/Linux ( Compressed ) - Mega, MixDrop, PixelDrain Updated the Android version of First and Second Acts with bug fixes. Updated Android version of Third Act to version 0.3.2. Also, the open-world events of the First and Second Acts have been removed. Added one more gold scene to the gallery. Improved and reworked dialog and images for the Wednesday starting events. Added a feature of tracking completed secondary and open-world events in the phone hints. Added a Level 1 secondary event for Makima. Added a Love Level 4 open-world event for Hinata and updated the open-world dialogs. You can also download it here or on itch.io ( )ģ80+ new renders, 70+ new animations, 2800+ new lines Hello, today Waifu Slut School 0.3.2 update became available to everyone! Loot the crate for potions and leave the cave. Take off everything from Tal before you fight them, if you don't, you lose some equipment and some potential gold. Not a problem as it is 3 vs 4 as Yarra joined you. After that, take on the Cache Guardian and get access to the stored power of corruption.Īfterwards, beat the bandits. Don't worry, she will not kill you even if you don't attack. The crate closest to the top contains five Antidotes, while the other contains a Collar. Move the rocks down and right, the second group left, then right and then up. Go east to the bridge, then go north to the cave. In the next area check the skeleton for some equipment. Fortunately, you don't have to prevent him from being knocked out. Of your 3 party members, Tal has the worst defense, agility, luck, and attack, even with the shining sword, so keep that in mind if you want him up and fighting. Experience in this game is finite so you should fight everything unless there's some reason not to, and Ivala's blessing gives you bonus EXP.Īn old man in the camp gives you a quest to collect mushrooms, which you can view on the Quests page. You should do the same with Altina's.) Some random plant monster is milling around, but you should first go east to recruit Aka. (Only available if you had cleared his inventory before the final fight of the Prologue. You should swap his Iron Sword for Kai's Shining Sword and the rest of his gear. Exit the temple and walk west to move to a new location. Go to the left room to start a easy training fight and open the chest with a basic set of armor (about same as current, but usable on other characters). Go to the right room and speak to the priestess to check your soul. Report the death of the Chosen and be stuck with a new one. When you wake up with full health, go east to the Church. Go to your house (the one with a padlock) get intercepted by your neighbor and rest. Instead, you (as Simon) will go through some cutscenes and get thrown to Feroholm.Ĭhapter 1: Soldier Ĭhosen assignment Return and report Chosen's death It seems that you're not the main character of this story. You can't beat Riala, so accept your fate and prepare for your death.Įxcept again, you were foiled and won't get a Game Over screen. except when you finally get to the room, Riala intercepts you. Remove all equipment from the members of your party and enter the final room. You will take them over when you beat the King. Inside are Orcs and Succubi, optional fights. Beat her, get another 'sex' scene, and enter the Fortress. Then kill the rest and claim an Iron Helm. Optional: if you go South from the Fortress, you will fight three Corrupted Bats, a Corrupted Spider and a Junior Succubus). Ice and Physical attacks hurt her the most. Try to stun her with Simon, it makes beating her easier as she applies Lust Stun effect. They are not monsters, they are more civil than humans at times. Go east to get your first actual fight against a mon. Further north-west is another chest with 3 Revival Potions and an optional battle against a spider. Loot the chest to get 5 Health Potions, 3 Mana Potions and 3 Revival Potions. Here you will find the weakness, resistances and in some cases some misc notes for the enemies of this area: Whatever you choose does not matter as they will gladly follow you to their doom. Altina is the mage and Simon is the only one who is not affected by Lust. Pretty straightforward, go north to meet Altina and Simon, your disposable servants. So it's not the intention of this page to give you a predefined roadmap with all the decisions already established, but you should find the all the relevant info in this page and many others, all of them linked from here. There is a good number of decisions where there is not an evident best option (and in many other cases, the best option will be only evident much later when it is known, you will be warned at the time). Despite the appearances, there is no such thing as an optimized playthrough for the game at this stage of the development process. The humor really stands out in this title. The lack of accuracy causes hilarious fails that will have you laughing as you prepare for another run. The harder selection of levels require pinpoint accuracy when landing, and a steady pace when jumping across the map. While the controls are easy to learn, mastering them will not be as simple. Fortunately, there aren’t too many situations that require you to know any advanced controls or mechanics. They aren’t complicated, but they are a bit finicky. You play the character specified when selecting the level, and earn points towards unlocking the sections containing other characters.Īs you play, you’ll quickly learn the controls. There are only six characters, and not all of them are available for every map. But, even ones that give you the freedom to choose a character are limited by the small selection available. Some game modes will require you to play a specific character. While the levels are not restricted, your character choices are. This is great because most games require some sort of progression to unlock certain levels. You have access to all levels, regardless of whether it’s the first one created or the last one. When you jump into the game for the first time you’ll find that there are no restrictions on what you can play. The blood and organs getting strewn about make a case for adult supervision while children play. While this gore may be fun, it isn’t suitable for younger audiences. Sometimes all that’s left of your character is their heart, making for a funny and uneasy moment. Simply jumping up and down can send a character flying, or you could quickly lose control as you’re racing towards a victory.Īs you play the game you’ll notice how your character falls apart. This is prevalent in many sections where the ragdoll mechanic gets used. While the levels are fun to play, the in-game physics are weird. You could explore a renaissance fair, throw knives and balls to gain points, or watch your body run through an intricately designed “do nothing” course. The created levels are, many times, well made, with a lot of attention to detail. Here, anyone can create and submit levels. Happy Wheels is a physics-based ragdoll adventure game where players ride through maps as one of many characters. It’s fun, silly and an ideal way to pass a boring afternoon. Play the happiest, most cheerful game on earth that’s filled with gore and an endless supply of pre-made levels. No specific protein bands visible.ĭoes the primary antibody recognize the protein of interest?Ĭheck with the manufacturer of the primary antibody that it has been validated for detection of protein or epitope tag of interest by Western blot. Make sure to expel air from between the gel and membrane before transfer. The Ponceau stain will identify bubbles which have interfered with protein transfer, and appear as blank circles among the transferred proteins. Small proteins can sometimes be captured by the addition of a second membrane when blotting.Īre air bubbles trapped between the gel and the membrane when blotting? If it is suspected that large proteins have not successfully transferred from gel to membrane, Coomassie stain may be used to detect proteins remaining in the acrylamide gel. Optimization of blotting conditions may be required, reducing or increasing the duration of transfer depending on the size of proteins to be blotted. Small proteins may pass through the membrane or large proteins may fail to transfer from gel to membrane. Although Ponceau cannot be used to identify a specific protein of interest, the presence of many faint pink/red bands on the blotting membrane confirms that proteins have been separated through the gel and have transferred onto the membrane. To confirm the transfer of proteins from the gel onto the blotting membrane, Ponceau S reversible stain can be a used before the blocking step. If not, then the transfer of the proteins from the electrophoresis gel to blotting membrane may have been unsuccessful. The prestained protein marker or ladder should be visible on the membrane after transfer. No bands are visible on the blotting membraneĬan the protein marker be seen on the membrane? If the Western blot is not behaving as expected, our troubleshooting guide may help isolate the problem. The robust nature of the antigen-antibody interaction allows the presence of specific proteins and peptides to be detected from complex mixtures. Dr Ralf J.Download PDF Western blotting is a staple technique of the molecular biology lab. They are prompt in replying to my emails and very helpful with their advice. Thanks a lot! Vahan Serobyan, Prof. provided by Diagenode to perform western blots.The high level of specificity of these antibodies in Pristionchus pacificus samples, confirmed by using several negative and positive controls run in parallel with synchronized culture samples, ensured successful and reproducible results.Īlso, I am satisfied with the service and the support of personnel of Diagenode. I have used the antibodies against the histone modifications H3K4me3, H3K4me2, H3K4me1, H3k27me3, H3K9ac, H3K27ac etc. I have been a Diagenode customer for over two years now. The high sensitivity and specificity of our antibodies enable the most accurate results. New! Blue ladder monoclonal antibody is available Antibodies validated in WBĭiagenode offers the antibodies selected for highest-quality results in Western blot. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. The left figure shows a Ponceau staining of the gel. Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody Outstanding specificity with no cross-reactivity or background.Compatible with most blue-stained ladders.Develop your marker directly on X-ray film.Faster and easier protein detection - manual marking is no longer necessary.This makes the positioning of the marker and thus the accurate detection of proteins significantly easier. Diagenode has developed a revolutionary new antibody that specifically reacts with this blue dye, enabling direct visualization of the different marker fragments on the blot. Most prestained protein MW markers used in western blot contain fragments that are labelled with a blue dye. Learn more about this antibody Blue ladder-HRP monoclonal antibody Visualize your marker directly on film To check that the signal detection after antibody incubation is homogenous across the lines.To confirm that the electrotransfer was done equally across the lines.To verify that equal sample amount was loaded on the gel.The marker (in kDa) is shown on the left,and the position of the protein of interest is indicated on the right. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Western blot was performed on whole cell extracts (30 μg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using the monoclonal antibody against H3. Western blot analysis using H3pan monoclonal antibody Classical players - or at least the pros and near-pros (i.e., students of the top players) - tend to be conservative regarding instrument appearance. If anyone has any advice or suggestions please let me know before I start looking at a Yamaha for classical playing! LOLĬlick to expand.The looks of a horn should never matter in determining how well it will work in a given genre of music, but the regrettable fact is that they sometimes seem to. I don't know whether to suck it up with my Eastman sax, or just use the Cannonball for everything and give my Eastman up. I have believed that the Cannonball is primarily a jazz horn but after trying my classical setup and it 's actually not that bad but I cant bring myself to accept using an extreme looking jazz horn for classical playing. Or it may be the fact that I dont my play classical setup as much and just not used to it. The problem just may be the mouthpiece and reed setup altogether which is why I am considering to try Vandoren Optimum and Selmer Concept mouthpieces. Both saxes give the dark classical sound I like but I don't know why it's resistant on my Eastman, especially since it got serviced a few months ago. The problem I am facing is that my classical mouthpiece setup is resistant and hard to blow through on my Eastman and is a little better on my Cannonball with the regular neck. For Jazz, I use the JodyJazz SuperJet 6 with BSS 2.5 reeds with bthe Fat Neck on the Cannonball, and I use a Selmer S80 C* with Vandoren V21 3 reeds on my Eastman. I started using the Cannonball for maily jazz and funk and the Eastman for classical playing. After I graduated, I upgraded to a Cannonball Big Bell Stone Series 25th Anniversary Edition Alto which I absolutely love. I have a Eastman 52nd Street Alto Sax that I used through my entire time at college until I graduated last year. Thank you all for the advice and suggestions!īefore I get into this, let me tell you about my equipment. Either that or buy the gear online and return what I don’t like. I will be trying different mouthpiece and reed combinations soon once I find a music store that has what I wanna try in stock. I got it serviced a few months ago at a regular music store and the tech must’ve missed that area so I’m gonna do some research to see where I can find another tech. The cork on the neck is thin so the mouthpiece wiggles around unless I push in all the way, and all the notes from low F down to low Bb become resistant so some of the keys still leak. I tested my jazz setup on my Eastman and that horn is the culprit. Focus on Original Content.EDIT: I have gathered that the looks of the saxophone doesn’t really matter. Keep it to 2 posts in a 24 hour period.īreak the cycle. Excessive posting to the sub will result in a ban.This sub is semi-official, and as such we have to conduct ourselves accordingly.Īrt posts that are obvious attempts to generate sales will be removed. Please refrain from self-promotion or sells in anyway. (exceptions to be made for certain holidays and special events like cons and official events) Szechuan Sauce, The word "Szechuan" that you saw somewhere - perhaps on a Chinese Restaurant sign or menu.Some chicken nuggets you found on the floor.Any memes, shitposts, captionposts, etc.Stuff you saw IRL that reminds you of something you saw from Rick and Morty.Low-effort posts, memes and anything that qualifies as a Shitpost now belongs in r/Mortytown.Įxamples include (but are not limited to): Unverified premiere dates & posts linking to clickbait/fake rumors are DISQUALIFIEDĨ. Posts and comments including personal information are DISQUALIFIEDĦ. Comment threads that dissolve into political shit-flinging will be locked.ĥ. Overtly Politically loaded posts are DISQUALIFIED. Posting leaks to any unreleased content is a bannable offense.ģ.Posts & comments directly linking to torrents are DISQUALIFIED. Please report any concerning behavior to the mods.Ģ. (Ban length depends on the severity of the asshole behavior).Ĭomment threads that dissolve into creepy behavior & shit-flinging will be locked and repeat offenders will be banned. Harassment, witchhunts, personal attacks and threats are bannable offenses. Heated discussions and normal arguments are fine, but please remember there’s a person on the other side of the screen. If you're looking for the shitposts, head over to the district over in /r/Mortytownġ. įor Episode Discussion Posts, check out our Wiki This is the subreddit for fans of Dan Harmon & Justin Roiland's animated series Rick and Morty on. Please review the sidebar rules before posting! Content that violates the rules will be DISQUALIFIED and removed at the mods' discretion. Artist Credit & Vendor Sourcing: Edit your post's flair to include a twitter/IG handle or post a separate comment in your thread with the relevant info They give you enough space for limited professional experience that otherwise would look like something’s missing. Single-column resumes are ideal for junior positions. Modern Web Developer Word Resume Template It’ll add some flair but also make your work history stand out, drawing focus to your most prominent professional work achievements. But what if your area of expertise isn’t designing resumes? This UX designer resume template for Word is the answer.Ī unique-colored sidebar can do wonders for your application if you do it right. Now, a professional UI/UX designer knows what great design is. Modern UI/UX Designer Word Doc Resume Template We recommend it for mid-level and senior employees.Įxpert Hint: Want to learn more about creating great resume objectives and summaries? 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Also, don't forget that Staubach had to serve in Vietnam before playing in the NFL, so he was a 27-year-old rookie. Aikman was only top five in these categories once each, all in 1992. For comparison, Staubach was in the top five in yards in six seasons, the top five in completions in four seasons and the top five in touchdowns in four seasons. The 1977 Dallas Cowboys, coached by Tom Landry, won the Super Bowl after finishing the NFL regular season in 1st place in the NFC East with a 12-2 record. Aikman did win three Super Bowls and was a very good quarterback with the Cowboys, but Staubach was a much bigger factor in his team's success. The 1977 Dallas Cowboys, coached by Tom Landry, won the Super Bowl after finishing the NFL regular season in 1st place in the NFC East with a 12-2 rec. He was named to the Hall of Fame All-1970s Team and inducted into the Hall of Fame in 1985. Super Bowl Champions (2nd championship) Scored 24.6 PPG (2nd) Allowed 15.1 PPG (8th) Team & Player Stats Stats Roster Roster Schedule & Results Schedule Splits Splits. The 1976 Dallas Cowboys, coached by Tom Landry, lost the Division Championship after finishing the NFL regular season in 1st place in the NFC East wi. Staubach was named the 1971 Bert Bell Player of the Year and the 1971 Super Bowl MVP. The 1977 Dallas Cowboys team and player stats for the NFL regular season and playoffs. Stories, Photos, Videos, Podcasts, and Publications featuring Dallas Cowboys 1976 Season. He led the NFL in touchdowns thrown in 1973, yards per attempt in 19 and quarterback rating in 1971, 1973, 19. Staubach gets the edge over Aikman for being the main offensive weapon for the Cowboys who made it to four Super Bowls in the 1970s, winning two. Roger Staubach is the greatest quarterback in Cowboys history, sorry Troy Aikman. This one was easier than you might think. They must be thorough and contain every project your team intends to work on in the near and distant future. Your budget enables you to coordinate marketing tactics with organizational objectives and direct funding toward marketing initiatives that provide the best ROI (ROI).īudgets for marketing are often created quarterly or annually by businesses. And depending on the sector, the region, and the company’s objectives, marketing spending might vary greatly.Ī marketing budget details the precise sums allotted for employee salaries, office costs, equipment purchases, marketing communications, ad design, and certain marketing channels. Setting a marketing budget can be difficult, particularly if you have never made marketing efforts. It will increase the profitability of your business so carefully plan your marketing budget.Every corporate marketing strategy should contain a marketing budget or the amount of money the company will spend to sell its products and services. It will help you to design your marketing strategies to successfully get the attention of the target market.
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